Reduction of Acetylene to Ethylene by Soybean Root Nodules
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چکیده
منابع مشابه
Reduction of acetylene and nitrogen gas by breis and cell-free extracts of soybean root nodules.
Cell-free extracts of Clostridiiumii p(lste'rtr'i lm il (8) aind( of A2otoboctcr viiell(tlfdii (2) in the presence of an ATP generating system alndI anl appropriate electron donior, catalvze the redcuction of N. gas to ammonia. In recent investigations (3,4, 9) of the properties of nitrogenase from these organiisms, it has been established that the enzyme system is non-specific for electron acc...
متن کاملAnalysis of acetylene reduction rates of soybean nodules at low acetylene concentrations.
It has been previously proposed that acetylene reduction data at subsaturating acetylene concentrations could be interpreted by use of the Michaelis-Menten equation, based on the acetylene concentration external to the nodules. One difficulty of this view is that the assumption that the system is not diffusion limited is violated when studying intact nodules. The presence of a gas diffusion bar...
متن کاملAn electron donor system for nitrogenase-dependent acetylene reduction by extracts of soybean nodules.
The catalysis of nitrogen reduction by extracts of nitrogen-fixing organisms requires an ATPgenerating system and a source of electrons (1, 2, 5, 11, 13). The phosplhoroclastic reaction provides botlh ATP anid electrons for the reduction of nitrogen by cell-free extracts of Clostridium1t pasteurianumn (2, 5, 13) and ferredoxin functions as the natural electron carrier from the phlosphoroclastic...
متن کاملPeptidohydrolases of Soybean Root Nodules
Nodule extracts prepared from Glycine max var Woodworth possessed endopeptidase, aminoptidase, and carboxypeptidase actiities. Three distinct endopeptidase activities could be resolved by disc-gel electrophoresis at pH 8.8. According to their order of increasing electrophoretic mobility, the first of these enzymes hydrolyzed azocasein and n-benzoyl-LLeu-p8-naphthylamide, while the second hydrol...
متن کاملStudies with detached lupin root nodules in culture: I. Maintenance and induction of acetylene reduction activity.
A method has been developed for culturing detached nitrogen-fixing root nodules of lupin (Lupinus angustifolius L.) on a simple nutrient medium. Under the best conditions devised, the acetylene reduction activity of mature detached nodules was maintained at 10 to 25 nmoles of ethylene hr(-1) mg(-1) fresh weight for 3 days. Under the same culture conditions, immature nodules increased their acet...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1966
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.41.10.1748